CST, 57932T, Lck (F7E4A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Lck mouse. Validated for WB,WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Lck (F7E4A) Rabbit Monoclonal Antibody (CST #57932) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Lck (F7E4A) Rabbit Monoclonal Antibody recognizes endogenous levels of total Lck protein. Non-specific nuclear labeling may be observed by immunofluorescence in fixed frozen mouse brain. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser32 of mouse Lck protein. Background Lck belongs to the Src-like non-receptor tyrosine kinase family with the typical Src family kinase structure: a unique amino terminal domain (Src homology 4 domain, SH4) followed by an SH3 domain, an SH2 domain, a kinase domain (SH1), and a carboxy-terminal negative regulatory domain (1). Lck activity is controlled by the interactions of SH2 and SH3 domains as well as tyrosine phosphorylation status of the activation loop (2,3). Lck is recruited to the T cell receptor (TCR) complex upon stimulation and activates downstream tyrosine kinases to initiate T cell signaling (4). Lck is also found to be involved in the regulation of mitochondrial apoptosis pathways and may be responsible for some anticancer drug induced apoptosis (5,6). Alternate Names Hck-3; Lck; Leukocyte C-terminal Src kinase; LSK; Lsk-t; Lskt; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; OTTMUSP00000010178; OTTMUSP00000010179; p56-LCK; p56; p56Lck; Proto-oncogene tyrosine-protein kinase LCK; RP23-209C6.8 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG