CST, 58069S, ChREBP Antibody

Polyclonal Antibody for studying ChREBP. Validated for Western Blotting. Highly specific and rigorously validated in-house, ChREBP Antibody (CST #58069) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ChREBP Antibody recognizes endogenous levels of total ChREBP protein. Based on amino acid sequence comparisons, the antibody is predicted to detect all known isoforms of ChREBP. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu308 of human ChREBP isoform α protein. The sequence surrounding this region is 100% conserved in all known isoforms of ChREBP. Antibodies are purified by protein A and peptide affinity chromatography. Background Carbohydrate-responsive element-binding protein (ChREBP) is a glucose-responsive transcription factor that regulates glycolytic and lipogenic gene expression (1). High levels of glucose induce the transcriptional activity of ChREBP. ChREBP is most abundant in tissues of lipogenesis and forms a heterotetramer with its binding partner MLX to bind to the promoter regions of its target genes (2). ChREBP regulates fatty acid synthesis, glucose homeostasis and insulin sensitivity in white adipose tissue. In white adipose tissue, ChREBP isoform α (ChREBP-α) activates the expression of the potent ChREBP isoform β (ChREBP-β) in a glucose-dependent manner. β expression levels predict insulin sensitivity in human white adipose tissue (1). In addition, research studies have shown that Akt2 induces the transcriptional activity of ChREBP-β to stimulate lipogenesis in brown adipose tissue for fuel storage (3). Furthermore, ChREBP-β is a potent activator of lipogenesis in the liver (4). Alternate Names BHLHD14; carbohydrate response element binding protein; Carbohydrate-responsive element-binding protein; CHREBP; Class D basic helix-loop-helix protein 14; MIO; MLX; MLX interacting protein like; MLX interacting protein-like; MLX interactor; MLX-interacting protein-like; MLXIPL; MLXPL; MONDOB; WBS14; WBSCR14; Williams Beuren syndrome chromosome region 14; Williams-Beuren syndrome chromosomal region 14 protein; Williams-Beuren syndrome chromosome region 14 protein 1; Williams-Beuren syndrome chromosome region 14 protein 2; Williams-Beuren syndrome chromosome region 14 protein 3; WS basic-helix-loop-helix leucine zipper protein; WS-bHLH Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit