CST, 58118S, NLRP4 (F7T1G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NALP4. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRP4 (F7T1G) Rabbit Monoclonal Antibody (CST #58118) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRP4 (F7T1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu814 of human NLRP4 protein. Background NOD-like receptor (NLR) family pyrin domain containing 4 (NLRP4), also known as NALP4, is a cytosolic protein with roles in regulating both autophagy, through interactions with beclin-1, and the type I interferon response by targeting TBK1 for degradation (1,2). NLRP4 also serves as an intracellular sensor for microbes and it is recruited to phagosomes containing group A where it associates with Rho GTPases and Rho GDP dissociation inhibitor alpha (ARHGDIA) to promote xenophagy (3). NLRP4 may also play a role in cancer, as non-small cell lung cancer patients with mutations displayed improved response to immune checkpoint blockade (4). In mice, knockdown led to enhanced type I interferon production and slower tumor growth (4). Conversely, upregulation enhances DNA damage repair in pancreatic cancer cell lines, and higher expression negatively correlates with mouse BxPC-3 xenograft response to olaparib treatment (5). Alternate Names Cancer/testis antigen 58; CLR19.5; CT58; FLJ32126; NACHT, leucine rich repeat and PYD containing 4; NACHT, LRR and PYD containing protein 4; NACHT, LRR and PYD domains-containing protein 4; NALP4; NLR family pyrin domain containing 4; NLR family, pyrin domain containing 4; NLRP4; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 4; PAAD and NACHT-containing protein 2; PAN2; PYPAF4; PYRIN and NACHT-containing protein 2; PYRIN-containing APAF1-like protein 4; Ribonuclease inhibitor 2; RNH2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 113 Source/Isotype: Rabbit IgG