CST, 58145S, CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD10 mouse. Validated for WB,WB,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (CST #58145) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67330 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD10/Neprilysin protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu493 of mouse CD10/Neprilysin protein. Background CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10 GPR77 carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4). Alternate Names 6030454K05Rik; Atriopeptidase; C85356; CALLA; CD10; common acute lymphoblastic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; Mme; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG