CST, 58173S, SH2D1B/EAT-2 (E2P5P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EAT-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SH2D1B/EAT-2 (E2P5P) Rabbit Monoclonal Antibody (CST #58173) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SH2D1B/EAT-2 (E2P5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total SH2D1B/EAT-2 protein. This antibody does not cross-react with SH2D1A/SAP protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly92 of human SH2D1B/EAT-2 protein. Background SH2 domain-containing protein 1B/EWS/FLI1-activated transcript 2 (SH2D1B/EAT-2) belongs to the SAP family of cytoplasmic signaling adaptor proteins. Structurally, SH2D1B/EAT-2 is highlighted by a single SH2 domain and a short C-terminus containing multiple tyrosine residues whose phosphorylation regulate SH2D1B/EAT-2 function (1,2). Research studies have demonstrated that SH2D1B/EAT-2 is recruited to multiple SLAM family receptors in a phosphotyrosine-dependent manner and that phosphorylation of its C-terminal tyrosine residues is required for control of downstream signaling (3-5). SH2D1B/EAT-2 expression has been detected in multiple hematopoietic cell lineages, including NK cells and macrophages (4,6). Indeed, in murine NK cells, tyrosine phosphorylation of SH2D1B/EAT-2 mediates inhibitory signaling downstream of 2B4/SLAMF4 to suppress effector functions (4). Alternate Names EAT-2; EAT2; EWS/FLI1-activated transcript 2; SH2 domain containing 1B; SH2 domain-containing molecule EAT2; SH2 domain-containing protein 1B; SH21B; SH2D1B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG