CST, 58189T, Tau (E5G6B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tau mouse. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Tau (E5G6B) Rabbit Monoclonal Antibody (CST #58189) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53737 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity Tau (E5G6B) Rabbit Monoclonal Antibody recognizes endogenous levels of total tau isoforms 5 and 8 protein (tau 2N). This antibody may detect the rodent tau 1N isoform. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val64 of mouse tau protein. Background Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Alternate Names AI413597; AW045860; Mapt; Microtubule-associated protein tau; Mta; Mtapt; Neurofibrillary tangle protein; OTTMUSP00000003078; OTTMUSP00000003083; Paired helical filament-tau; PHF-tau; Tau Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50-80 Source/Isotype: Rabbit IgG