CST, 58203S, Dinitrophenol (D1D6) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, Dinitrophenol (D1D6) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #58203) is ready to ship. Product Usage Information Western Blotting: 1:10000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Dinitrophenol (D1D6) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes dinitrophenol-conjugated molecules. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with KLH-conjugated dinitrophenol. Background The synthetic compound dinitrophenol (DNP) is a small hapten molecule that can be conjugated to proteins for use in immunoassays (1). Antibodies that recognize dinitrophenol will display strong binding affinity to DNP conjugated molecules, including nucleic acids and proteins. These DNP-conjugated compounds can be used in hybridization, western, northern, and Southern blotting (1,2). Dinitrophenol is a commonly used insecticide and has been used as a treatment for weight loss (3). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG