CST, 58207T, SOX11 (E7G9N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SOX11. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, SOX11 (E7G9N) Rabbit Monoclonal Antibody (CST #58207) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SOX11 (E7G9N) Rabbit Monoclonal Antibody recognizes endogenous levels of total SOX11 protein. In some cell extracts, the antibody weakly detects a 48 kDa protein. The identity of this protein is unknown. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro323 of human SOX11 protein. Background SRY-related high mobility group box (SOX) proteins comprise a large family of widely conserved transcription factors that play important roles in development. SOX proteins possess a high mobility group (HMG) motif that binds the DNA minor groove. SOX proteins typically do not mediate transcription directly, but require binding partners that regulate their ability to mediate transcription of target genes that control cell fate determination and development (reviewed in 1). SOX11 is generally described as a neural transcription factor, whose expression is critical for the establishment of neuronal identity in the embryonic mammalian brain (2). It has furthermore been reported to function as a pioneer transcription factor, able to bind nucleosomes and influence the transcription of genes in regions of closed chromatin, in similar fashion to its close relative SOX2 (3). Postnatal expression of SOX11 is reported to be uncommon, but aberrant SOX11 expression has been reported in numerous malignancies, including mantle cell lymphoma, Burkitt's lymphoma, and various lymphoblastic leukemias (4-6). Alternate Names CSS9; MRD27; SOX11; SRY (sex determining region Y)-box 11; SRY (sex-determining region Y)-box 11; SRY-box 11; SRY-box transcription factor 11; SRY-related HMG-box gene 11; Transcription factor SOX-11 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG