CST, 58208S, Caspase-12 (E9T3W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp12 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-12 (E9T3W) Rabbit Monoclonal Antibody (CST #58208) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspase-12 (E9T3W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Caspase-12 protein. This antibody detects the large subunit of Caspase-12 associated with activation. Bands of unknown identity were observed at 42 and 30 kDa. These may represent additional caspase-12 isoforms. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a region within the large subunit of mouse Caspase-12 protein. Background Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER stress-induced apoptosis, such as high calcium concentration, low oxygen, and low glucose levels (1,2). One of the mechanisms for caspase-12 activation is related to calpain-mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of caspase-12 (2,3). Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein (3). In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process (1). Alternate Names CASP-12; Casp12; caspase 12; Caspase-12; caspase12; CASPC Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 55, 38, 28 Source/Isotype: Rabbit IgG