CST, 58225T, CD62L/L-Selectin (E7X9I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying L-Selectin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD62L/L-Selectin (E7X9I) Rabbit Monoclonal Antibody (CST #58225) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98131 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD62L/L-Selectin (E7X9I) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD62L/L-Selectin protein. Non-specific staining was observed in the surface epithelium of tonsil by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human CD62L/L-Selectin recombinant protein. Background L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6). Due to cell type-specific glycosylation, the actual molecular weight of L-Selectin differs between cell types and can range from 60 kDa in lymphocytes up to 100 kDa in neutrophils (7). Alternate Names CD62 antigen-like family member L; CD62L; gp90-MEL; L-selectin; LAM-1; LAM1; LECAM1; LEU8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; LYAM1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-100 Source/Isotype: Rabbit IgG