CST, 58258S, PSPH (E1D2X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PSPH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PSPH (E1D2X) Rabbit Monoclonal Antibody (CST #58258) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PSPH (E1D2X) Rabbit Monoclonal Antibody recognizes endogenous levels of total PSPH protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly141 of human PSPH protein. Background Serine biosynthesis involves sequential reactions catalyzed by several metabolic enzymes, including phosphoserine phosphatase (PSPH). PSPH catalyzes the dephosphorylation of phospho-L-serine to L-serine, the last irreversible step in serine biosynthesis (1-3). Studies show that is overexpressed in melanoma samples, and this overexpression is essential for tumor growth and metastasis. knockdown in melanoma cells leads to increased 2-hydroxyglutarate (2-HG) levels. 2-HG suppresses melanoma cell growth (3). Alternate Names L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; Phosphoserine phosphatase; PSP; PSPase; PSPH; PSPHD; SERB Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG