CST, 58289S, ELAVL1/HuR (D9W7E) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying ELAVL1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, ELAVL1/HuR (D9W7E) Rabbit Monoclonal Antibody (PE Conjugate) (CST #58289) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ELAVL1/HuR (D9W7E) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total ELAVL1/HuR protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His201 of human ELAVL1/HuR protein. Background The ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1/HuR, ELAVL2/HuB, ELAVL3/HuC and ELAVL4/HuD (1). ELAVL1/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3' UTRs to stabilize mRNAs (3, 4). ELAVL1/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1/HuR interacts with microRNAs (miRNAs) (7). Alternate Names ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV like RNA binding protein 1; ELAV-like protein 1; ELAV1; ELAVL1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; Hu-antigen R; Hua; HUR; MelG Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG