CST, 58309S, DDRGK1 (F6M1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDRGK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DDRGK1 (F6M1S) Rabbit Monoclonal Antibody (CST #58309) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DDRGK1 (F6M1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDRGK1 protein. Based on epitope mapping, this antibody is predicted to bind isoform 1 and not isoform 2 of DDRGK1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp244 of human DDRGK1 protein. Background The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4). DDRGK1 (also called UFBP1, C20orf116, and Dashurin) is an ER protein that is a key component of the UFMylation system and regulator of ER homeostasis (15,16). It is also a regulator of NF-κB activity (17,18). DDRGK1 is indispensable for embryonic development and hematopoiesis (17). Alternate Names C20orf116; Dashurin; DDRGK; DDRGK domain containing 1; DDRGK domain-containing protein 1; DDRGK1; dJ1187M17.3; MGC2592; SEMDSH; UFBP1; UFM1-binding and PCI domain-containing protein 1; UFM1-binding protein 1 containing a PCI domain Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG