CST, 5839S, SDHA Antibody

Polyclonal Antibody for studying SDHA. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, SDHA Antibody (CST #5839) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SDHA Antibody recognizes endogenous levels of total SDHA protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SDHA protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington's disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas (4,5). Alternate Names CMD1GG; DHSA; Flavoprotein subunit of complex II; Fp; FpSDH; PGL5; SDH1; SDH2; SDHA; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex flavoprotein subunit A; succinate dehydrogenase complex, subunit A, flavoprotein (Fp) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit