CST, 5846S, NDRG3 Antibody

Polyclonal Antibody for studying NDRG3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NDRG3 Antibody (CST #5846) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NDRG3 Antibody recognizes endogenous levels of total NDRG3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NDRG3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The NDRG (N-Myc downstream-regulated gene) family consisting of NDRG1, NDRG2, NDRG3, and NDRG4 are structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was upregulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues and have been suggested to function as a tumor suppressors (4,5). Members of the NDRG family each have distinct expression patterns; NDRG3 is most highly expressed in the testis, prostate, ovary, and brain (2,6,7). NDRG3 has also been shown to be up-regulated by androgen in prostate cell lines and can promote cell growth in those cells (8). Alternate Names FLJ13556; N-myc downstream-regulated gene 3; N-myc downstream-regulated gene 3 protein; NDRG family member 3; NDRG3; Protein NDRG3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 45 SOURCE: Rabbit