CST, 5847S, RecQL5 (1A2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying RECQL5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RecQL5 (1A2) Mouse Monoclonal Antibody (CST #5847) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RecQL5 (1A2) Mouse Monoclonal Antibody recognizes endogenous levels of total RecQL5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human RecQL5 protein. Background The RecQ family is a group of DNA helicases that play an important role in global genomic stability (1). Mutations in three of the five known human RecQ proteins (BLM, WRN, and RECQL4) give rise to clinically distinct disorders that are characterized by features such as premature aging and predisposition to cancer (2,3). The clinical distinction of each disease associated with these mutations points to distinct roles that members of this helicase family play in DNA metabolism. The RecQL5 helicase has not yet been associated with any human disease, but RecQL5 -/- mice exhibit an increased incidence of cancer (4,5). It has recently been shown that RecQL5 protects genome stability through two parallel mechanims: helicase action and interaction with the initiation form of RNA Polymerase II (6). It has also been shown that RecQL5 -/- mouse embryonic stem cells display an elevated frequency of sister chromatic exchange (SCE), suggesting a role in suppression of homologous recombination and/or crossover events (7,8). Alternate Names ATP-dependent DNA helicase Q5; DNA helicase, RecQ-like type 5; FLJ90603; RecQ helicase-like 5; RecQ like helicase 5; RecQ protein 5; RecQ protein-like 5; RECQ5; RECQL5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Mouse IgG1