CST, 5848T, Brd2 (D89B4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BRD2. Validated for WB,WB,ChIP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, Brd2 (D89B4) Rabbit Monoclonal Antibody (CST #5848) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP, Chromatin IP-seq, CUT&RUN Specificity / Sensitivity Brd2 (D89B4) Rabbit Monoclonal Antibody recognizes endogenous levels of total Brd2 protein. This antibody may cross-react with Brd3 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala310 of human Brd2 protein. Background Brd2 is a highly conserved member of the BET subfamily of bromodomain proteins that contain two tandem N-terminal bromodomains and a single C-terminal extra-terminal (ET) domain (1). In addition to its involvement in guiding the expression of cell cycle genes through its binding to multiple E2Fs (2), Brd2 has been shown to be associated with several regulators of transcription, including TFIID and Swi/Snf complexes (3,4). First identified as a nuclear serine/threonine kinase (2), Brd2, like other bromodomain proteins, is thought to function in mammalian development by regulating chromatin structure and transcription (5). Brd2 has been shown to bind to histone H4 via acetylated Lys12, a substrate of several histone acetyltransferase transcriptional coactivators (6). In mouse, Brd2 has the highest levels of expression during embryogenesis and in the adult testis, ovaries, and brain (3,7,8). Brd2-deficient mouse embryos exhibit delayed development and eventual death due to neural tube closure defects (5). Mutations in the promoter of the gene have been associated with increased susceptibility to juvenile myoclonic epilepsy (JME) (9). Alternate Names BRD2; BRD2 intronic transcript 1; BRD2-IT1; bromodomain containing 2; bromodomain-containing 2; Bromodomain-containing protein 2; D6S113E; DKFZp686N0336; female sterile homeotic-related gene 1; FLJ31942; FSH; FSRG1; KIAA9001; NAT; O27.1.1; Really interesting new gene 3 protein; RING3; RNF3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG