CST, 5849S, GFI1b (D3G2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GFI1B. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, GFI1b (D3G2) Rabbit Monoclonal Antibody (CST #5849) is ready to ship. Product Usage Information Western Blotting: 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity GFI1b (D3G2) Rabbit Monoclonal Antibody recognizes endogenous levels of total GFI1b protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr119 of human GFI1b protein. Background GFI1b and its homolog GFI1 are transcriptional repressors and important regulators of erythroid and megakaryocytic development and differentiation (1,2). GFI1b negatively regulates transcription by recruiting chromatin regulatory proteins including CoREST, the histone demethylase LSD1 and HDACs 1 and 2, which associate with GFI1b via its SNAG repression domain (3). GFI1b has also been shown to control the differentiation of erythroid and megakaryocytic progenitors by regulating TGF-β signaling at the bipotent progenitor stage (4). Inactivation of GFI1b in mice leads to embryonic lethality due to failure to produce functional erythrocytes and megakaryocytes (2). The GFI1b gene locus can be autoregulated by binding to its own promoter in hematopoietic cells, likely through interacting with GATA-1, another transcription factor essential for erythroid and megakaryocytic development (5). Mutations in GFI1b are implicated in various leukemias (6) and GFI1b has been found in a complex with GATA-1 and SUZ12 on repressed genes in erythroleukemia cells (7). Alternate Names BDPLT17; GFI1B; growth factor independent 1B (potential regulator of CDKN1A, translocated in CML); growth factor independent 1B transcription repressor; growth factor independent 1B transcriptional repressor; Growth factor independent protein 1B; Potential regulator of CDKN1A translocated in CML; Zinc finger protein Gfi-1b; ZNF163B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 35, 42 Source/Isotype: Rabbit IgG