CST, 5850S, Sall4 Antibody

Polyclonal Antibody for studying SALL4. Validated for Western Blotting. Highly specific and rigorously validated in-house, Sall4 Antibody (CST #5850) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Sall4 Antibody recognizes endogenous levels of total Sall4 protein, isoforms A and B. This antibody may also detect other Sall4 isoforms. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Sall4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Members of the gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in have been implicated in human malformation syndromes, including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7). Alternate Names dJ1112F19.1; DRRS; HSAL4; MGC133050; sal-like 4 (Drosophila); Sal-like protein 4; SALL4; spalt like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80, 142 SOURCE: Rabbit