CST, 5852S, GATA-3 (D13C9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GATA3. Validated for WB,WB,IHC,IF,F,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, GATA-3 (D13C9) Rabbit Monoclonal Antibody (CST #5852) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:1600 - 1:6400 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Chromatin IP: 1:100 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN Specificity / Sensitivity GATA-3 (D13C9) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATA-3 protein. This antibody is not recommended for immunofluorescence analysis of frozen mouse tissues due to non-specific staining. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr63 of human GATA-3 protein. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12). Alternate Names GATA binding protein 3; GATA-binding factor 3; GATA3; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG