CST, 5853S, HO-1 (D60G11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HO-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HO-1 (D60G11) Rabbit Monoclonal Antibody (CST #5853) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HO-1 (D60G11) Rabbit Monoclonal Antibody recognizes endogenous levels of total HO-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala110 of human HO-1 protein. Background Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory, and cytoprotective functions (2). HO comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer's disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5). Alternate Names bK286B10; heat shock protein, 32-kD; heme oxygenase (decycling) 1; Heme oxygenase 1; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG