CST, 5854T, WTX/AMER1 (D38E5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WTX. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, WTX/AMER1 (D38E5) Rabbit Monoclonal Antibody (CST #5854) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity WTX/AMER1 (D38E5) Rabbit Monoclonal Antibody detects endogenous levels of total WTX/AMER1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human WTX/AMER1 protein. Background WTX (Wilms' tumor gene on the X chromosome) is a developmentally regulated gene with a potentially important role in kidney development (1). Functional studies of WTX suggest that it acts as a tumor suppressor gene in renal cells by promoting β-catenin ubiquitination and degradation, thereby antagonizing WNT/β-catenin signaling (1,2). WTX is found to be inactivated in 30% of Wilms' tumors, mostly by chromosomal deletion (3). Wilms' tumor is a pediatric kidney cancer that arises from cells that fail to differentiate during kidney development (4). Inactivation of the WT1 tumor suppressor gene accounts for 10-15% of Wilms' tumor cases (5). WTX has been shown to enhance WT1-mediated transactivation, suggesting a physiologically significant interaction between WT1 and WTX (1). WTX may be directly involved in the transcriptional regulation of cellular differentiation in the kidney through interactions with WT1 and other transcription factors (1). Alternate Names adenomatous polyposis coli membrane recruitment 1; AMER1; APC membrane recruitment protein 1; F123B; FAM123B; family with sequence similarity 123B; FLJ39827; OSCS; Protein FAM123B; RP11-403E24.2; Wilms tumor gene on the X chromosome protein; Wilms tumor on the X; WTX Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit