CST, 58595S, MAP1B Antibody

Polyclonal Antibody for studying MAP1B. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, MAP1B Antibody (CST #58595) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:3200 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MAP1B Antibody recognizes endogenous levels of total MAP1B protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1570 of human MAP1B protein. Antibodies are purified by peptide affinity chromatography. Background Microtubule-associated protein 1B (MAP1B) is a member of the high molecular weight microtubule binding phosphoprotein family, along with MAP1A (1). MAP1B is highly expressed in neurons, predominantly during development, where it regulates axonal guidance and elongation through its influence on microtubule dynamics (1,2). In mature neurons, MAP1B plays a role in regulation and structure of dendritic spines in glutamatergic synapses. MAP1B deficient neurons have been shown to display structural presynaptic deficiencies, including a decrease in density at synaptic terminals and disruption of synaptic vesicle fusion events (2). Mutations in have been linked to developmental disorders affecting cognitive ability through a deficit in white matter and compromised axons (3). Alternate Names DKFZp686E1099; DKFZp686F1345; FLJ38954; FUTSCH; MAP-1B; MAP1 light chain LC1; MAP1B; MAP1B heavy chain; MAP5; microtubule associated protein 1B; Microtubule-associated protein 1B; PPP1R102; protein phosphatase 1, regulatory subunit 102; PVNH9 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 270 SOURCE: Rabbit