CST, 5868T, KAP-1 (4E1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying TIF1b. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, KAP-1 (4E1) Mouse Monoclonal Antibody (CST #5868) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity KAP-1 (4E1) Mouse Monoclonal Antibody recognizes endogenous levels of total KAP-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human KAP-1 protein. Background KRAB-associated protein 1 (KAP-1) is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs - an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). KAP-1 is a corepressor for KRAB (Kruppel associated box) domain-containing zinc finger proteins. The KRAB domain containing zinc finger proteins are a large group of transcription factors that are vertebrate-specific, varied in their expression patterns between species, and thought to regulate gene transcription programs that control speciation (3,4). KAP-1 has been shown to be essential for early embryonic development and spermatogenesis (6,5). It functions to either activate or repress transcription in response to environmental or developmental signals by chromatin remodeling and histone modification. The recruitment and association of KAP-1 with heterochromatin protein (HP1) is essential for transcriptional repression and for progression through differentiation of F9 embryonic carcinoma cells (6,7). KAP-1 also plays a role in the DNA damage response. Phosphorylation of KAP-1 on Ser824 occurs in an ATM-dependent manner in response to genotoxic stress and is thought to be essential for chromatin relaxation, which is, in turn, required for the DNA damage response (8). Alternate Names E3 SUMO-protein ligase TRIM28; FLJ29029; KAP-1; KAP1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; TIF1-beta; TIF1B; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; TRIM28; tripartite motif containing 28; tripartite motif-containing 28; Tripartite motif-containing protein 28 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100, 75 Source/Isotype: Mouse IgG1