CST, 5871S, Protease Inhibitor Cocktail (100X)

Buffer for studying in the research area. Product Usage Information 1. Briefly vortex the Phosphatase Inhibitor Cocktail (100X) before use. 2. Just prior to lysing cells, dilute the cocktail 1:100 in desired lysis buffer to obtain a 1X working concentration. Note: If EDTA is desired as a protease inhibitor it can be added to the cell lysis buffer at a final working concentration of 5 mM. Storage Store the undiluted 100X cocktail at 4ºC. Do not freeze. Background In order to study specific target proteins of interest protease-mediated degradation during the generation of protein lysates is to be avoided. A loss of normal cellular control occurs during cell lysis, and endogenous proteases within the cell extract are free to degrade proteins in an uncontrolled manner. The addition of protease inhibitors to the cell lysis buffer aids in the preservation of target proteins in the cell extract. Alternate Names aebsf; aprotinin; bestatin; cocktail; E-64; e64; leupeptin; pepstatinA; Protease Inhibitor Cocktail; Protease Inhibitors; tablet