CST, 58802S, Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) Monoclonal Antibody (HRP Conjugate)

Secondary Antibody for studying Mouse IgG in the research area. Product Usage Information Recommended Antibody Dilution: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) Monoclonal Antibody (HRP Conjugate) recognizes native mouse IgG. This antibody conjugate also recognizes the denatured and reduced mouse IgG light chains (about 25 kDa) on a western blot. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with native total mouse IgG. Background Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) only reacts with native IgG and denatured and reduced mouse IgG light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG heavy chains of the primary antibody used for IP run at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) as a secondary antibody will eliminate this problem. Alternate Names IgG; mouse IgG Specification REACTIVITY: M Source/Isotype: Rabbit IgG