CST, 5880T, eNOS (6H2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying eNOS. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, eNOS (6H2) Mouse Monoclonal Antibody (CST #5880) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 26339 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity eNOS (6H2) Mouse Monoclonal Antibody recognizes endogenous levels of total eNOS protein. Species Reactivity: Human, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human eNOS protein. Background Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H O activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). Alternate Names cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII Specification REACTIVITY: H B SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Mouse IgG1