CST, 5882S, STAG2 (D25A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STAG2. Validated for WB,IP,IF,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, STAG2 (D25A4) Rabbit Monoclonal Antibody (CST #5882) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity STAG2 (D25A4) Rabbit Monoclonal Antibody recognizes endogenous levels of total STAG2 protein. This antibody does not cross-react with other STAG proteins, including STAG1 and STAG3. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe21 of human STAG2 protein. Background The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Alternate Names bA517O1.1; Cohesin subunit SA-2; DKFZp686P168; DKFZp781H1753; FLJ25871; HPE13; MKMS; NEDXCF; SA-2; SA2; SCC3 homolog 2; SCC3B; STAG2; Stromal antigen 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 141 Source/Isotype: Rabbit IgG