CST, 58844S, Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tyrosine Hydroxylase. Validated for WB,IHC,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody (CST #58844) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:150 - 1:600 Immunohistochemistry (Paraffin): 1:150 - 1:600 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 81242 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody recognizes endogenous levels of total tyrosine hydroxylase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human tyrosine hydroxylase protein. Background Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and post-translational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31, and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). Alternate Names dystonia 14; DYT14; DYT5b; HTH-2; TH; TH2; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55-60 Source/Isotype: Rabbit IgG