CST, 5885S, Phospho-Desmoplakin (Ser165/166) Antibody

Polyclonal Antibody for studying Desmoplakin (Ser165/Ser166) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Desmoplakin (Ser165/166) Antibody (CST #5885) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Desmoplakin (Ser165/166) Antibody recognizes endogenous levels of desmoplakin protein only when phosphorylated at Ser165/166. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser165/166 of human desmoplakin protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Desmosomes are a class of intracellular junctions that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein desmoplakin, which acts as a tether by binding the cytoplasmic component of the desmosome at its N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8). Phospho-Desmoplakin (Ser165/166) Antibody is directed against a site that was identified at Cell Signaling Technology (CST) using PTMScan , our LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus , a modification site knowledgebase developed and maintained by CST, at www.phosphosite.org for more information. Alternate Names 250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; Desmoplakin; desmoplakin I; desmoplakin II; DESP; DP; DPI; DPII; DSP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 320, 250 SOURCE: Rabbit