CST, 58865SF, COL14A1 (E5W8S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying COL14A1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, COL14A1 (E5W8S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #58865) is ready to ship. Product Usage Information This product is the carrier free version of product #61964. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 61964 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity COL14A1 (E5W8S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total COL14A1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COL14A1 protein. Background COL14A1 (Collagen Type XIV, Undulin) is an interstitial collagen that belongs to the fibril-associated collagens with interrupted triple helices (FACIT) family (1). COL14A1 contributes to the assembly and remodeling of the interstitial extracellular matrix, in part by promoting the formation of inter-molecular bridges between collagen molecules (2-4). Changes in COL14A1 expression have been identified as a potential biomarker in a variety of fibrotic diseases, including idiopathic pulmonary fibrosis (5,6) and fibrotic kidney diseases (7). A study of tumor stroma-associated biomarkers furthermore revealed that was one of a group of genes whose expression correlated negatively with clinical stage of breast cancer patient samples (8). Alternate Names COEA1; COL14A1; Collagen alpha-1(XIV) chain; collagen type XIV alpha 1 chain; collagen, type XIV, alpha 1; UND; Undulin; undulin (fibronectin-tenascin-related) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit IgG