CST, 58891T, HoxA10 (E5X4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HOXA10. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HoxA10 (E5X4L) Rabbit Monoclonal Antibody (CST #58891) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HoxA10 (E5X4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total HoxA10 protein. This antibody detects a band of unknown origin around 32 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu188 of human HoxA10 protein. Background HOXA10 is a member of the HOXA cluster which, along with the HOXB, HOXC, and HOXD clusters, governs embryonic patterning along the cranio-caudal axis (1,2). HOXA10 was originally identified in hematopoietic cells, where it is alternatively spliced into two isoforms, the larger being expressed in early stages of myeloid differentiation (2,3). Expression of HOXA10 can be regulated by estrogen and progesterone and is important for uterine implantation (4-6). HOXA10 is a transcription factor that partners with Pbx proteins to bind DNA, and this process can be affected by tyrosine phosphorylation (7,8). Overexpression of HOXA10 has been shown to contribute to acute myeloid leukemia and is associated with poor prognosis (9,10). Alternate Names homeo box A10; homeobox A10; homeobox protein 1H; Homeobox protein Hox-1.8; Homeobox protein Hox-1H; Homeobox protein Hox-A10; homeobox protein HOXA10; HOX1; HOX1.8; HOX1H; HOXA10; HXA10; MGC12859; PL Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG