CST, 5890S, SOS1 Antibody

Polyclonal Antibody for studying SOS1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SOS1 Antibody (CST #5890) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SOS1 Antibody detects endogenous levels of total SOS1 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile1198 of human SOS1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Son of sevenless (SOS) was first identified in as a guanine nucleotide exchange factor (GEF) for Ras acting downstream of the Sevenless receptor (1). Two closely related homologs of SOS are found in mammalian cells: SOS1 and SOS2 (2). SOS1 consists of histone folds, Dbl (DH) and pleckstrin (PH) homology domains, a Ras exchange motif (REM), and Cdc25 homology and polyproline domains (3). SOS1 binds to GRB2, NCK, and other adaptor proteins, and plays an important role in ERK activation downstream of protein tyrosine kinase receptor (RTK). Research studies have identified mutations in the corresponding SOS1 gene of patients with Noonan syndrome, a developmental disorder characterized by short stature, facial dysmorphia, and congenital heart defects (4,5). Alternate Names GF1; GGF1; GINGF; gingival fibromatosis, hereditary, 1; guanine nucleotide exchange factor; HGF; NS4; Son of sevenless homolog 1; son of sevenless homolog 1 (Drosophila); SOS Ras/Rac guanine nucleotide exchange factor 1; SOS-1; SOS1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 152 SOURCE: Rabbit