CST, 58935S, TCPTP (TC45) (D7T7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PTPN2 iso2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TCPTP (TC45) (D7T7D) Rabbit Monoclonal Antibody (CST #58935) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 29857 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TCPTP (TC45) (D7T7D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TCPTP (TC45) protein. This antibody does not recognize the 48 kDa (TC48) isoform. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of human TCPTP (TC45) protein. Background T cell protein tyrosine phosphatase (TCPTP, PTPN2, PTN2) is a non-receptor protein tyrosine phosphatase (PTP) that regulates signal transduction pathways by catalyzing the dephosphorylation of tyrosine residues (1). Two described TCPTP splice variants include a 48 kDa isoform (TC48) that is targeted to secretory pathway organelles (e.g., endoplasmic reticulum) by a hydrophobic carboxy terminus, and a 45 kDa isoform (TC45) that actively shuttles between the nucleus and cytoplasm (2). TCPTP substrates include receptor and non-receptor tyrosine kinases, such as EGFR, JAK1/3, and Src-family kinases, as well as STAT3 and other nuclear substrates (3-6). Research studies show that the corresponding gene is deleted in a subset of human T-cell acute lymphoblastic leukemias. The loss of TCPTP has been suggested to promote tumor progression through enhanced JAK/STAT signaling (7,8). Alternate Names protein tyrosine phosphatase non-receptor type 2; protein tyrosine phosphatase, non-receptor type 2; PTN2; PTPN2; PTPN2 iso2; PTPT; T-cell protein tyrosine phosphatase; T-cell protein-tyrosine phosphatase; TC-PTP; TCELLPTP; TCPTP; Tyrosine-protein phosphatase non-receptor type 2 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG