CST, 58990SF, Rab27A (D7Z9Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying RAB27A. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Rab27A (D7Z9Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #58990) is ready to ship. Product Usage Information This product is the carrier free version of product #69295. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 69295 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Rab27A (D7Z9Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Rab27a protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab27a protein. Background Rab27 is a member of the Ras superfamily of small Rab GTPases implicated in exocytosis (1-2). The protein is localized in secretory lysosomes, such as melanosomes in melanocyte or lytic granules in cytotoxic T cells to control exosome secretion pathway (3-5). Rab27 has two isoforms, Rab27a and Rab27b. Rab27a colocalizes with part of CD63 staining vesicles, and Rab27b shows perinuclear distribution. Target knock out studies indicate that the isoforms control different steps of the exosome secretion pathway (6). Rab27a interacts with a wide range of effectors and is involved in multiple steps of exocytosis depending on the effector it associated with and the cell type that is involved (1,2). Rab27a has been shown to be an important player in leukocyte function, cancer metastasis and invasion, and insulin secretion (7-11) Alternate Names GS2; GTP-binding protein Ram; HsT18676; MGC117246; mutant Ras-related protein Rab-27A; Rab-27; RAB27; RAB27A; RAB27A, member RAS oncogene family; RAM; Ras-related protein Rab-27A; RB27A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG