CST, 58992S, Peptide Desalting C18 StageTip Adaptor

Miscellaneous for studying in the research area. Product Usage Information Use with a Peptide Desalting C18 StageTip for processing peptide samples prior to liquid chromatography-mass spectrometry (LCMS). Dimensions of microcentrifuge tubes and centrifuge devices can vary, and we recommend a microcentrifuge tube that can accommodate the inner diameter of the adaptor and a centrifuge that can accommodate the tube, adaptor, and StageTip with a rotor lid. Below are examples of equipment we have tested that fit the provided adaptor. Recommended equipment (not provided): Posi-Click 1.5 mL graduated microcentrifuge tube (Thomas Scientific) Peptide Desalting C18 StageTip (Cell Signaling Technology, #45943) MiniSpin Centrifuge with F-45-12-11 rotor (Eppendorf) 1) Remove the lid from a clean 1.5 mL microcentrifuge tube. 2) Insert an adaptor inside the microcentrifuge tube. 3) Insert a Peptide Desalting C18 StageTip through the central hole in the adaptor. The StageTip should sit easily inside the adaptor. There should be enough space between the bottom of the microcentrifuge tube and the bottom of the StageTip to accommodate at least 100 μL of liquid. Users can proceed with peptide desalting. 4) After experiments are finished, users can wipe clean the adaptor with 70% ethanol and air dry it if necessary. DO NOT use detergent- or soap-containing solutions to clean the adaptor. Users can store the adaptor for re-use at room temperature in a closed bag or box to minimize dust exposure. Storage Store at room temperature.