CST, 59035S, beta2-microglobulin Antibody

Polyclonal Antibody for studying B2M mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, beta2-microglobulin Antibody (CST #59035) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity beta2-microglobulin Antibody recognizes endogenous levels of total β2-microglobulin protein. This antibody detects a 75 kDa band of unknown origin in some cell lines. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln49 of mouse β2-microglobulin protein. Antibodies are purified by peptide affinity chromatography. Background β2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). As an integral component of the MHC class I complex, β2-microglobulin plays a critically important role in immune system function (3). It has important relevance to cancer biology research; for example, research studies have shown that nearly one-third of diffuse large B cell lymphomas contain mutations that inactivate β gene function, thereby allowing tumor cells to escape immune detection (4). In addition, β2-microglobulin has been identified as an amyloid preprotein with collagen-binding affinity (5); its accumulation in osteoarthritic lesions of long-term dialysis patients is reportedly a contributing factor to the condition known as amyloid osteoarthropathy (6). Alternate Names B2m; B2MG; beta-2 microglobulin; Beta-2-microglobulin; beta2-; beta2-m; beta2m; Ly-m1; Ly-m11; OTTMUSP00000016550 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 12 SOURCE: Rabbit