CST, 59036S, OX40L (D6K7R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OX40L. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, OX40L (D6K7R) Rabbit Monoclonal Antibody (CST #59036) is ready to ship. Product Usage Information IHC Leica Bond: 1:200 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 51635 . Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity OX40L (D6K7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total OX40L protein. Staining of unknown specificity has been observed in skeletal muscle. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human OX40L protein. Background OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12). Alternate Names CD134 ligand; CD134L; CD252; Glycoprotein Gp34; GP34; OX-40L; OX40 antigen ligand; OX40 ligand; OX40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); TNF superfamily member 4; TNFL4; TNFSF4; TNLG2B; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand 2B; Tumor necrosis factor ligand superfamily member 4; tumor necrosis factor superfamily member 4; TXGP1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG