CST, 59115SF, GDF15/MIC1 (E4D3K) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Mic-1/GDF15. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),ELISA+. Highly specific and rigorously validated in-house, GDF15/MIC1 (E4D3K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #59115) is ready to ship. Product Usage Information This product is the carrier free version of product #48481. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 48481 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GDF15/MIC1 (E4D3K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GDF15/MIC1 protein. This antibody reacts with monomeric and dimeric forms of pro-GDF15/MIC1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human GDF15/MIC1 protein. Background Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-β (2), PLAB (3), PDF (4), and NAG-1 (5), is a divergent member of the transforming growth factor-β (TGF-β) superfamily (6). Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site, generating an active C-terminal domain that is secreted as a dimeric protein. Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer. In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7). It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9). During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenesis, and metastasis, and may also contribute to weight loss during cancer (10,11). Alternate Names GDF-15; GDF15; growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC1; NAG-1; non-steroidal anti-inflammatory drug-activated gene-1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta; PTGFB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG