CST, 59170S, IL-18 (E3G8R) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying IL18. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL-18 (E3G8R) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #59170) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IL-18 (E3G8R) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human IL-18 recombinant protein. Background Interleukin-18 (IL-18), originally known as interferon-gamma inducing factor (IGIF), is part of the IL-superfamily of cytokines (1,2). This proinflammatory cytokine is synthesized as an inactive precursor which requires cleavage by Caspase-1 to become an active, mature molecule, which is secreted by monocytes and macrophages (3,4,6). It induces IFNγ production in T-helper-1 (Th1) cells and natural killer (NK) cells, and can regulate innate immunity via both Th1 and Th2 responses (4,5). Elevated IL-18 levels are associated with autoimmune disease, but may be balanced by binding to IL-18 binding protein (IL-18BP), which along with IL-18 neutralizing antibodies, are being examined in clinical trials (6,7). Alternate Names Iboctadekin; IFN-gamma-inducing factor; IGIF; IL 18; IL-1 gamma; IL-18; IL-1g; IL18; IL1F4; Interferon gamma-inducing factor; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; MGC12320 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG