CST, 59172T, FBP1/FBPase 1 (D2T7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FBPase. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FBP1/FBPase 1 (D2T7F) Rabbit Monoclonal Antibody (CST #59172) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FBP1/FBPase 1 (D2T7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total FBP1/FBPase 1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val106 of human FBP1/FBPase 1 protein. Background Fructose-1,6-bisphosphatase 1 (FBP1 or FBPase 1), a rate limiting enzyme in gluconeogenesis, catalyzes the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate (1). Inhibition of expression in basal-like breast cancer (BLBC) cells leads to metabolic reprogramming, including enhanced glycolysis, which leads to increased glucose uptake, biosynthesis of macromolecules, and activation of PKM2 (1). This metabolic reprogramming endows tumor cells with cancer stem cell (CSC)-like properties, thereby increasing their tumorigenicity (1). Depletion of FBP1 was also reported in more than 600 clear cell renal cell carcinoma (ccRCC) tumors, suggesting that FBP1 may inhibit ccRCC tumor progression (2). Alternate Names D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; F16P1; FBP; FBP1; FBPase; FBPase 1; Fructose-1,6-bisphosphatase 1; fructose-bisphosphatase 1; growth-inhibiting protein 17; liver FBPase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 39 Source/Isotype: Rabbit IgG