CST, 59242S, Orexin Receptor 1/HCRTR1 (E5Q8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HCRTR1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Orexin Receptor 1/HCRTR1 (E5Q8B) Rabbit Monoclonal Antibody (CST #59242) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Orexin Receptor 1/HCRTR1 (E5Q8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total Orexin Receptor 1/HCRTR1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala9 of human Orexin Receptor 1/HCRTR1 protein. Background Orexin Receptor 1 or Hypocretin G-protein-coupled receptor-1 (OX1R and HCRTR1, respectively) is 1 of 2 orexin receptors expressed in the brain. Neurons in the hypothalamus express orexin receptors that bind to neuropeptides to modulate neuronal function (1,2). The neuropeptides that bind orexin receptors include Orexin-A (hypocretin-1) and Orexin-B (hypocretin-2). Orexin-A binds with a higher affinity to HCRTR1, whereas Orexin-B binds more selectively to HCRTR2 (2). Activation of HCRTR1 by its cognate neuropeptide drives downstream signaling events regulating several cellular processes, including increases in cytoplasmic Ca levels (2,3). HCRTR1 has been linked to many behavioral processes, including wakefulness as well as migraines and mood disorders (4). The link of the orexin system to sleep and mood disorders make it a potential therapeutic target to combat insomnia, depression, anxiety, and addiction (3).   Alternate Names HCRTR1; hypocretin (orexin) receptor 1; hypocretin receptor 1; Hypocretin receptor type 1; hypocretin receptor-1; orexin receptor 1; Orexin receptor type 1; orexin receptor-1; Orexin/Hypocretin receptor type 1; Ox-1-R; Ox1-R; Ox1R Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43-52 Source/Isotype: Rabbit IgG