CST, 5926S, DHX29 (D19E10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DHX29. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DHX29 (D19E10) Rabbit Monoclonal Antibody (CST #5926) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DHX29 (D19E10) Rabbit Monoclonal Antibody recognizes endogenous levels of total DHX29 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DHX29 protein. Background DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2). Alternate Names ATP-dependent RNA helicase DHX29; DDX29; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29; DEAH (Asp-Glu-Ala-His) box polypeptide 29; DEAH box protein 29; DEAH-box helicase 29; DExH-box helicase 29; DHX29; FLJ21492; Nucleic acid helicase DDXx Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 155 Source/Isotype: Rabbit IgG