CST, 59278T, DDX21 (E9O2Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDX21. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DDX21 (E9O2Q) Rabbit Monoclonal Antibody (CST #59278) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DDX21 (E9O2Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX21 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala780 of human DDX21 protein. Background DDX21, also known as RNA helicase II/Gu, is an RNA helicase that plays important roles in transcription and ribosomal RNA (rRNA) processing (1-3). DDX21 associates with many RNA Polymerase I and II genes, including the direct binding to the rDNA locus to promote ribosome biogenesis. The helicase activity of DDX21 contributes to transcription by helping release P-TEFb from the 7SK small nuclear ribonucleoprotein (snRNP) complex (3). Nucleolar localization of DDX21 has been shown to be regulated through phosphorylation by JNK and interaction with c-Jun. Depletion of either c-Jun or JNK has shown to impair rRNA processing (4,5). DDX21 can also become acetylated by CBP, which reduces its activity. Deacetylation by SIRT7 increases DDX21 helicase activity to prevent R-loop accumulation and protect genomic stability (6). DDX21 can also form a complex with DDX1, DHX36, and TRIF to sense dsRNA, which in turn activates interferon response in innate immunity (7,8). Alternate Names DDX21; DEAD (Asp-Glu-Ala-Asp) box helicase 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD box protein 21; DEAD-box helicase 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; DExD-box helicase 21; DKFZp686F21172; Gu protein; Gu-alpha; GUA; GURDB; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu; RH-II/GU; RH-II/GuA; RNA helicase II/Gu alpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG