CST, 59283SF, LSR (D3E3N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LISCH/LSR/ILDR3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, LSR (D3E3N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #59283) is ready to ship. Product Usage Information This product is the carrier free version of product #14804. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14804 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LSR (D3E3N) XP Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly449 of human LSR protein. Background The lipolysis-stimulated lipoprotein receptor (LSR, LISCH) is an immunoglobulin superfamily member and single pass transmembrane protein that binds the apolipoprotein B (ApoB) and apolipoprotein E (ApoE) lipoproteins (1). LSR is responsible for the cellular uptake of triacylglyceride-rich lipoproteins and supports lipid distribution between the liver and peripheral tissues (1,2). The LSR protein is expressed at the cell membrane as a heterodimer consisting of α and β subunits, which are produced as alternative splice variants from a single gene (3). Research studies suggest that LSR acts as the host cell surface receptor for multiple toxins (4) and participates in the formation of tricellular tight junctions in epithelial cells (5). Additional studies demonstrate that LSR expression is up-regulated in several cancer types, including breast, bladder, and colorectal cancer, which could lead to pro-tumorigenic changes in metabolism (6-8). Alternate Names angulin-1; ILDR3; immunoglobulin-like domain containing receptor 3; lipolysis stimulated lipoprotein receptor; Lipolysis-stimulated lipoprotein receptor; lipolysis-stimulated receptor; lipolysis-stimulated remnant; LISCH; LISCH protein; LISCH7; liver-specific bHLH-Zip transcription factor; LSR; MGC10659; MGC48312; MGC48503 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG