CST, 59286T, GNLY (E1V5M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GNLY. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, GNLY (E1V5M) Rabbit Monoclonal Antibody (CST #59286) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 91378 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity GNLY (E1V5M) Rabbit Monoclonal Antibody recognizes endogenous levels of total GNLY protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GNLY protein. Background Granulysin (GNLY) was originally identified as a late activation marker in T cells, and it is expressed by killer lymphocytes in most mammals, but not rodents. GNLY is largely confined to cytotoxic granules but can be secreted by killer cells, especially those expressing high levels of GNLY, such as decidual natural killer cells (1-3). GNLY is produced as a 15 kDa protein and is processed into a 9 kDa active pore-forming fragment by proteolytic removal of peptides from both the N- and C-termini. Moreover, the 15 kDa form was reported to function as an immune alarmin, causing the maturation and migration of antigen-presenting cells and other cells of the immune system (4-7). Unlike perforin, a cholesterol-dependent pore-forming protein that preferentially permeabilizes mammalian membranes, GNLY is inhibited by cholesterol and forms pores much more efficiently in microbial than mammalian membranes, and it plays an important role against bacteria, fungi, and parasite infections (8,9). Alternate Names D2S69E; GNLY; Granulysin; LAG-2; LAG2; lymphocyte-activation gene 2; Lymphokine LAG-2; NKG5; Protein NKG5; T-cell activation protein 519; T-lymphocyte activation gene 519; TLA519 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG