CST, 5929S, TBC1D1 (G689) Antibody

Polyclonal Antibody for studying TBC1D1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TBC1D1 (G689) Antibody (CST #5929) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TBC1D1 (G689) Antibody detects endogenous levels of total TBC1D1 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Gly 689 of human TBC1D1. Antibodies are purified by protein A and peptide affinity chromatography. Background TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified in skeletal muscle (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5). Alternate Names KIAA1108; TBC; TBC1; TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1; TBC1 domain family member 1; TBC1D1; TBCD1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit