CST, 59309S, Leupaxin (D4I3B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying leupaxin. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Leupaxin (D4I3B) Rabbit Monoclonal Antibody (CST #59309) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Leupaxin (D4I3B) Rabbit Monoclonal Antibody recognizes endogenous levels of total leupaxin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg35 of human leupaxin protein. Background Leupaxin is a member of the paxillin family of scaffold proteins whose functions include regulation of cell adhesion, migration and metastasis (1). Leupaxin suppresses integrin-dependent tyrosine phosphorylation of paxillin and affects the regulation of cell-matrix adhesion (2). Researchers have implicated leupaxin in the progression of human hepatocellular carcinoma through its regulation of beta-catenin-mediated transcription (3). Researchers have also shown that leupaxin regulates actin dynamics and migration of prostate cancer cells through its interaction with caldesmon (4) and down-regulation of p120-catenin (5). Studies in breast cancer implicate leupaxin in the regulation of ER-alpha transcription and invasiveness (6). Alternate Names LDLP; LDPL; Leupaxin; LPXN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG