CST, 59333T, Crry (E9F1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Crry mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Crry (E9F1S) Rabbit Monoclonal Antibody (CST #59333) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Crry (E9F1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Crry protein. This antibody cross-reacts with unidentified proteins between 25-30 kDa in some cell extracts. This antibody is not recommended for immunohistochemical analysis of human tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse Crry protein. Background Crry, also known as complement component receptor 1-like protein (Cr1l), is a murine membrane-bound complement regulatory protein ubiquitously expressed in mouse tissues (1,2). Crry is a functional analog of the human complement decay-accelerating factor (DAF/CD55) and membrane cofactor protein (MCP/CD46) (3). In mice, Crry acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissues (4,5). Crry also acts as a decay-accelerating factor, preventing the formation of C4b2a and C3bBb, which are amplification convertases of the complement cascade (3,6). Crry plays a crucial role in early embryonic development by maintaining fetomaternal tolerance (7). On CD4 T cells, Crry acts as a costimulatory factor which favors IL-4 rather than IFN-gamma secretion, suggesting Th2 polarization (8). Cancer cells and cells of the tumor microenvironment have been shown to overexpress Crry, suppressing tumor-specific T cell responses and complement-mediated anti-tumor immunity (9,10). Multiple isoforms have been identified computationally. Alternate Names complement component (3b/4b) receptor 1-like; Complement component receptor 1-like protein; complement receptor related protein; Complement regulatory protein Crry; Cr1l; Crry; Cry; Mcp; mCR; mCRY; MGC102484; Protein p65 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG