CST, 59346S, VISTA (D1L2G) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying VISTA. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, VISTA (D1L2G) Rabbit Monoclonal Antibody (Alexa Fluor^® 555 Conjugate) (CST #59346) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity VISTA (D1L2G) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VISTA protein. Background VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7). Alternate Names B7-H5; B7H5; C10orf54; DD1alpha; Death Domain1alpha; Dies1; GI24; PD-1H; PDCD1 homolog; Platelet receptor Gi24; PP2135; Sisp-1; SISP1; stress induced secreted protein 1; Stress-induced secreted protein-1; V-domain Ig suppressor of T cell activation; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation; VISTA; VSIR Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG